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GSG1 , a yeast gene required for sporulation
Author(s) -
Kaytor Michael D.,
Livingston Dennis M.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111205
Subject(s) - biology , gene , meiosis , genetics , open reading frame , saccharomyces cerevisiae , mutant , accession number (library science) , genbank , ploidy , yeast , microbiology and biotechnology , peptide sequence
We have identified a gene, GSG1 ( g eneral s porulation g ene 1), required for sporulation in Saccharomyces cerevisiae . Diploids homozygous for a disruption of GSG1 fail to sporulate. The gene has an open reading frame of 2094 bp, encoding a polypeptide with an expected size of 77 kDa. GSG1 is expressed mitotically in both a and α haploids, and both mitotically and meiotically in diploids. The message level of GSG1 increases approximately two‐fold after 4–6 h of sporulation. gsg1 mutants enter pre‐meiotic DNA synthesis later than wild‐type diploids. Mutant diploids are not rescued by spo13 . These results suggest that GSG1 has a role late in meiosis following DNA replication. The sequence reported here has the GenBank Accession Number U26674.