Premium
II . Yeast sequencing reports. Sequence analysis of a 78·6 kb segment of the left end of Saccharomyces cerevisiae chromosome II
Author(s) -
Obermaier Brigitte,
Gassenhuber Johann,
Piravandi Ester,
Domdey Horst
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111112
Subject(s) - orfs , biology , genetics , open reading frame , gene , nucleic acid sequence , subtelomere , saccharomyces cerevisiae , sequence analysis , accession number (library science) , chromosome , microbiology and biotechnology , peptide sequence , genbank
Abstract We report the sequence analysis of a 78,601 bp DNA segment on the left arm of chromosome II of Saccharomyces cerevisiae . This 78·6 kb segment spans the region from the start of a subtelomeric Y′ element up to the ILS1 gene. It contains 49 open reading frames (ORFs) with more than 100 amino acids length including 14 internal and five overlapping ORFs. The gene density, excluding the internal ORFs, was calculated as one ORF per 2·2 kb. Eight ORFs ( PKC1, TyA, TyB, ATP1, ROX3, RPL17a, PET112 and ILS1 ) correspond to previously characterized genes. ORF YBL0718 was identified as CDC27 ; YBL0706 as TEL1 . Four other ORFs show strong similarities to already known genes. The gene product of YBL0838 is 60% identical to the ribosomal protein RPL32 from rat, mouse and man. YBL0701 encodes a protein with significant similarity to the initiation factor eIF2 associated p67 glycoprotein from rat. Eight ORFs were disrupted and the resulting yeast strains analysed with respect to their phenotype. The sequence has been deposited in the EMBL Nucleotide Sequence Database under the Accession Number X79489.