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XVI . Yeast sequencing reports. A new essential gene GCR4 located in the upstream region of GCR1
Author(s) -
Uemura Hiroshi,
Jigami Yoshifumi
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111111
Subject(s) - biology , genbank , accession number (library science) , genetics , saccharomyces cerevisiae , gene , start codon , cloning (programming) , yeast , amino acid , base sequence , computer science , programming language
A new essential gene of Saccharomyces cerevisiae was found upstream of GCR1 . Its cloning and sequencing predict a 280 amino acid protein (32 577 Da). The predicted protein is fairly hydrophobic, and a search of the database did not identify any homologous proteins. A LEU2 disruption at codon 104 was lethal, but disruption at codon 221 showed a temperature‐sensitive conditional growth phenotype. Abnormalities were observed in some glycolytic enzyme levels. The sequence has been submitted to GenBank‐EMBL‐DDBJ under Accession Number D29645.