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Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps
Author(s) -
Cardinali Gianluigi,
Pellegrini Leandro,
Martini Alessandro
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111104
Subject(s) - biology , yeast , dna extraction , dna , genetics , chromosomal analysis , extraction (chemistry) , computational biology , chromosome , gene , chromatography , karyotype , polymerase chain reaction , chemistry
A modified procedure is proposed for chromosomal DNA extraction based on a cell‐wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non‐ Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.