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SSU71 , encoding the largest subunit of TFIIF, is located on the right arm of chromosome VII in Saccharomyces cerevisiae
Author(s) -
Sun ZuWen,
Hampsey Michael
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110811
Subject(s) - biology , genetics , gene , gene cluster , nucleic acid sequence , gene mapping , chromosome , microbiology and biotechnology
SSU71 (TFG1) is an essential nuclear gene encoding the largest subunit of the yeast general transcription factor TFIIF. The SSU71 gene was physically mapped to the right arm of chromosome VII, physically linked to QCR9 , by hybridization of the cloned gene to CHEF and lambda clone grid blots. This assignment was confirmed by genetic mapping. A search of the nucleotide sequence databases revealed that SSU71 is immediately adjacent to the TYS1 gene, which encodes tRNA Tyr synthetase. TYS1 was reported previously to lie on chromosome XV based on sequence overlap with the adjacent UBR1 gene. The mapping data reported here established that TYS1 and UBR1 do not lie on chromosome XV; rather the SSU71‐TYS1‐UBR1 gene cluster lies on the right arm of chromosome VII, physically linked to QCR9 and genetically linked to ade3 and ser2 .