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Host cell properties and external pH affect proinsulin production by Saccharomyces yeast
Author(s) -
Kozlov Dmitry G.,
Prahl Natalija,
Efremov Boris D.,
Peters Lars,
Benevolensky Sergei V.,
Wambut Rolf,
Karpychev Igor V.,
Eldarov Mikhail A.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110803
Subject(s) - biology , proinsulin , yeast , saccharomyces cerevisiae , affect (linguistics) , host (biology) , saccharomyces , genetics , microbiology and biotechnology , insulin , communication , sociology
Abstract The expression of a hybrid gene encoding an α‐factor prepro leader peptide‐miniproinsulin (MPI) fusion [MPI is the same as the LysArg human insulin precursor described by Thim et al. (1986)] was tested in a series of isogenic yeast strains to investigate the influence of some genetic and physiological factors on heterologous production in yeast. We found that: (i) an MFα1 gene disruption in haploid cells, as well as MFα1 gene product expression in diploid cells, do not affect the MPI secretion level; (ii) under conditions of exogenous leucine availability, MPI production is hindered by leucine auxotrophy (a leu2 mutation); (iii) rho − mutations increase the per‐cell MPI yield approximately three‐fold; (iv) the MPI yield is apparently dependent on the pH of the culture medium: the higher the external pH, the larger the per‐cell MPI yield.