Localization of the FAR3 gene: Genetic mapping and molecular cloning using a chromosome walk‐‘n’‐roll strategy

FAR3 is a newly‐discovered yeast gene required specifically for pheromone‐mediated cell cycle arrest. I have used strains harboring the far3‐1 mutation to map the gene to the right arm of chromosome XIII, establishing the gene order CEN13‐LYS7‐MCM1‐FAR3 . I cloned the FAR3 gene based on its genetic map position using a strategy that combined chromosome walking and a related technique termed ‘chromosome rolling’. In addition to the genetic and physical localization of FAR3 , I present data that suggest corrections to the tentative map positions of VAN1 and ARG80 .