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Sequence of a 9·8 kb segment of yeast chromosome II including the three genes of the MAL3 locus and three unidentified open reading frames
Author(s) -
Feuermann M.,
Charbonnel L.,
De Montigny J.,
Bloch J.C.,
Potier S.,
Souciet J.L.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110707
Subject(s) - biology , orfs , genetics , subtelomere , locus (genetics) , open reading frame , gene , maltase , saccharomyces cerevisiae , chromosome , pseudogene , peptide sequence , genome , biochemistry , enzyme
We report the DNA sequence of a segment located on the right arm of chromosome II from Saccharomyces cerevisiae S288C near the subtelomeric sequences. The sequence was determined using a random cloning strategy followed by an oligonucleotide‐directed sequencing. The segment contains four non‐overlapping open reading frames (ORFs) YBR297w, YBR298c, YBR299w and YBR301c, and two overlapping ones (YBR300c and YBR300w). Three of them—YBR297w, YBR298c and YBR299w—are the MAL3R (transcriptional regulatory protein), MAL3T (maltose permease) and MAL3S (maltase) genes of the MAL3 locus previously localized. The three other ORFs are unidentified. Another MAL locus ( MAL1 ) has been localized on chromosome VII. The Mal − phenotype of strain S288c cannot be explained by telomeric silencing. The sequences have been submitted to the EMBL data library under Accession Numbers Z36166; Z36167; Z36168; Z36169 and Z36171.