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The upstream sequences of the HSP82 and HSC82 genes of Saccharomyces cerevisiae : Regulatory elements and nucleosome positioning motifs
Author(s) -
Erkine Alexander M.,
SzentGyorgyi Christopher,
Simmons Serena F.,
Gross David S.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110607
Subject(s) - biology , saccharomyces cerevisiae , genetics , gene , nucleosome , upstream (networking) , computational biology , regulatory sequence , regulation of gene expression , histone , computer network , computer science
We present the upstream sequences of HSP82 and HSC82 , two closely related, but differentially regulated, heat‐shock genes of Saccharomyces cerevisiae . Several dozen potential regulatory elements are identified within each upstream region; interestingly, only a few are conserved between the two genes. These include a consensus heat‐shock element, an upstream repressor element, and a consensus TATA element. A search for motifs known actively to position nucleosomes in vitro revealed that such sequences are three‐ to seven‐fold enriched within each promoter; a comparable enrichment is seen near the 3′ end of each transcription unit. Located ∼ 1100 bp upstream of HSC82 is an open reading frame (ORF) of 255 amino acids; ∼ 800 bp upstream of HSP82 is an ORF of 132 amino acids. The latter ORF contains several conserved ankyrin motifs and appears to be expressed under normal growth conditions. Finally, we show by clamped homogeneous electric field gel electrophoresis that the two genetic loci map to different chromosomes: HSP82 to chromosome XVI and HSC82 to chromosome XIII. The sequences have been deposited in the GenBank database under Accession Numbers U20323 and U20349.