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Sequence analysis of a 5·6 kb fragment of chromosome II from Saccharomyces cerevisiae reveals two new open reading frames next to CDC28
Author(s) -
Baur Sabine,
Becker Jûrgen,
Li Ziyu,
Niegemann Eckhard,
Wehner Eugen,
Wolter Ralf,
Brendel Martin
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110508
Subject(s) - biology , open reading frame , genetics , saccharomyces cerevisiae , gene , accession number (library science) , orfs , nucleic acid sequence , chromosome , peptide sequence , microbiology and biotechnology , genbank
The sequence of a 5653 bp DNA fragment of the right arm of chromosome II of Saccharomyces cerevisiae contains two unknown open reading frames (YBR1212 and YBR1213) next to gene CDC28 . Gene disruption reveals both putative genes as non‐essential. ORF YBR1212 encodes a predicted protein with 71% similarity and 65% identity (total polypeptide of 376 aa) with the 378 aa Sur1 protein of S. cerevisiae , while the putative product of ORF YBR1213, which is strongly expressed, has 28% identity with a Lactococcus lactis ‐secreted 45 kDa protein and 24% identity with the Saccharomyces cerevisiae AGA1 gene product. The total sequence of the fragment has been submitted to the EMBL databank (accession number X80224).