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UAS NTR functioning in combination with other UAS elements underlies exceptional patterns of nitrogen regulation in Saccharomyces cerevisiae
Author(s) -
Rai Rajendra,
Daugherty Jon R.,
Cooper Terrance G.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110307
Subject(s) - biology , saccharomyces cerevisiae , computational biology , genetics , gene
UAS NTR , the UAS responsible for nitrogen catabolite repression‐sensitive transcriptional activation of many nitrogen catabolic genes in Saccharomyces cerevisiae , has been previously thought to operate only as a pair of closely related dodecanucleotide sites each containing the sequence GATAA at its core. Here we show that a single UAS NTR site is also able to combine with another unrelated cis ‐acting element to mediate transcription as well. In one instance the unrelated cis ‐acting element was TTTGTTTAC situated upstream of GLN1 , while in another the cis ‐acting element was the one previously shown to bind the PUT3 protein. When a UAS NTR site functions in combination with an unrelated site, the regulatory responses observed are a hybrid consisting of characteristics derived from both the UAS NTR site and the unrelated site as well. These observations resolve several significant inconsistencies that have plagued studies focused on elucidation of the mechanisms involved in the global regulation of nitrogen catabolism.