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Effects of phleomycin‐induced DNA damage on the fission yeast Schizosaccharomyces pombe cell cycle
Author(s) -
Belenguer Pascale,
Oustrin MarieLouise,
Tiraby Gérard,
Ducommun Bernard
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110305
Subject(s) - schizosaccharomyces pombe , biology , schizosaccharomyces , yeast , fission , cell cycle , dna , dna damage , genetics , microbiology and biotechnology , cell , saccharomyces cerevisiae , physics , quantum mechanics , neutron
Abstract The effect of phleomycin, a bleomycin‐like antibiotic, has been investigated in the fission yeast, Schizosaccharomyces pombe . We report that in response to phleomycin‐induced DNA damage, growth was inhibited and S. pombe cells arrested in the G2‐phase of the cell cycle. DNA repair mutants rad9 and rad17 did not arrest and were hypersensitive to phleomycin. Cell cycle mutants that entered mitosis without monitoring the completion of DNA replication also displayed an increased sensitivity to this DNA‐damaging agent. Thus, phleomycin could be used as a tool in the fission yeast S. pombe model system for the study of DNA damage and cell cycle checkpoints, or as a new selective agent.