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Transcriptional regulation of the Saccharomyces cerevisiae HXK1 , HXK2 and GLK1 genes
Author(s) -
Herrero P.,
Galíndez J.,
Ruiz N.,
MartínezCampa C.,
Moreno F.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320110205
Subject(s) - biology , saccharomyces cerevisiae , gene , transcriptional regulation , gene expression , psychological repression , messenger rna , enzyme , regulation of gene expression , glycolysis , biochemistry , microbiology and biotechnology
In Saccharomyces cerevisiae , the transcriptional regulation of most glycolytic genes has been extensively studied. By contrast, little is known about the transcriptional control of the three glucose‐phosphorylating enzymes, although this catalytic reaction has an important role in the regulation of cell metabolism. In this paper, we describe the transcriptional regulation of the HXK1, HXK2 and GLK1 genes in the hope of revealing differences in the steady‐state levels of mRNA associated with a particular carbon source used in the culture medium. Our results provide evidence supporting a differential expression of the three genes depending on the carbon source used for growth. We have also studied the induction and repression kinetics of mRNA expression for the HXK1, HXK2 and GLK1 genes.