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The nucleotide sequence and initial characterization of pyruvate decarboxylase from the yeast Hanseniaspora uvarum
Author(s) -
Holloway Paul,
Subden Ronald E.
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320101207
Subject(s) - pyruvate decarboxylase , biology , homotetramer , saccharomyces cerevisiae , gene , biochemistry , isozyme , yeast , nucleic acid sequence , enzyme , microbiology and biotechnology , structural gene , genetics , protein subunit , escherichia coli , alcohol dehydrogenase
We have isolated a pyruvate decarboxylase (PDC) gene from the yeast Hanseniaspora uvarum using the Saccharomyces cerevisiae PDC1 gene as a probe. The nucleotide sequence of this gene was determined and compared to PDC genes from yeast and other organisms. The H. uvarum PDC gene is more than 70% identical to the S. cerevisiae PDC isozymes and possesses a putative thiamine diphosphate binding site. The PDC enzyme was purified and partially characterized. The H. uvarum PDC was very similar to other known PDCs; the K m for pyruvate was 0·75 mM, and the enzyme is a homotetramer with subunits of M r = 57 000. The sequence has been submitted to GenBank under Accession No. U13635.