Premium
IX . Yeast sequencing reports. Cloning and sequence of REV7 , a gene whose function is required for DNA damage‐induced mutagenesis in Saccharomyces cerevisiae
Author(s) -
Torpey Laurence E.,
Gibbs Peter E. M.,
Nelson John,
Lawrence Christopher W.
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320101115
Subject(s) - biology , saccharomyces cerevisiae , mutagenesis , genetics , gene , complementation , mutant , genbank , dna , yeast , microbiology and biotechnology
The function of the REV7 gene is required for DNA damage‐induced mutagenesis in budding yeast, Saccharomyces cerevisiae , and is therefore thought to promote replication past sites of mutagen damage in the DNA template. We have cloned this gene by complementation of the rev7‐2 mutant defect, and determined its sequence. REV7 encodes a predicted protein of M r 28 759 which is unlike any other protein in the NCBI non‐redundant protein sequence data base, and which is inessential for viability. The sequence of the 3·88 kb yeast genomic fragment containing REV7 has been deposited in Genbank accession number U07228.