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Characterization of lipid particles of the yeast, Saccharomyces cerevisiae
Author(s) -
Leber Regina,
Zinser Erwin,
Paltauf Fritz,
Daum Gûnther,
Zellnig Gûnther
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320101105
Subject(s) - saccharomyces cerevisiae , organelle , yeast , sterol , biology , biochemistry , lipid droplet , antiserum , mutant , lipid bilayer , membrane , cholesterol , gene , antibody , immunology
Lipid particles of the yeast, Saccharomyces cerevisiae , were isolated to high purity and their components were analysed. The hydrophobic core of this organelle consists of triacylglycerols and steryl esters, which are almost exclusively located to that compartment. Lipid particles are stabilized by a surface membrane consisting of phospholipids and proteins. Electron microscopy confirmed the purity of the preparations and the proposed structure deduced from biochemical experiments. Major proteins of lipid particles have molecular weights of 72, 52, 43 and 34 kDa, respectively. The 43 kDa protein reacts with an antiserum against human apolipoprotein AII. In lipid particles of the yeast mutant strain S. cerevisiae erg6 , which is deficient in sterol Δ 24 ‐methyltransferase, this protein is missing thereby identifying the protein and confirming our previous finding (Zinser et al. , 1993) that sterol Δ 24 ‐methylation is associated with lipid particles. A possible involvement of surface proteins of lipid particles in the interaction with other organelles is discussed with respect to sterol translocation in yeast.