Physiological approach to heterologous human serum albumin production by  Kluyveromyces lactis  in chemostat culture | Zendy

Blondeau Karine | Zendy; Boze Hélène | Zendy; Jung G. | Zendy; Moulin G. | Zendy; Galzy P. | Zendy

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Physiological approach to heterologous human serum albumin production by Kluyveromyces lactis in chemostat culture

Author(s) -

Blondeau Karine,

Boze Hélène,

Jung G.,

Moulin G.,

Galzy P.

Publication year - 1994

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.320101006

Subject(s) - chemostat , heterologous , kluyveromyces lactis , biology , phosphoglycerate kinase , albumin , yeast , biochemistry , fermentation , kluyveromyces , recombinant dna , microbiology and biotechnology , saccharomyces cerevisiae , enzyme , bacteria , gene , genetics

Production of recombinant human serum albumin (rHSA) controlled by the constitutive promoter phosphoglycerate kinase was studied in Kluyveromyces lactis . It was governed by both cell concentration and glycolytic flow. The triggering of the fermentation metabolism by unfavourable culture conditions (pH, pO 2 , D ) caused a decrease in the synthesis of the heterologous protein. The highest productivity (75 mg 1 −1 per h) and rHSA concentration (62 mg 1 −1 ) were obtained in chemostat culture with a dilution rate of 0·12 h −1 and with 38 g 1 −1 dry weight.

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