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VII . Yeast sequencing reports. Cloning and characterization of a sulphite‐resistance gene of Saccharomyces cerevisiae
Author(s) -
Casalone Enrico,
Colella Carlo M.,
Daly Simona,
Fontana Silvia,
Torricelli Ilaria,
Polsinelli Mario
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100812
Subject(s) - biology , saccharomyces cerevisiae , gene , cloning (programming) , genetics , mutant , transversion , zinc finger , sequence analysis , nucleic acid sequence , molecular cloning , peptide sequence , mutation , microbiology and biotechnology , computer science , transcription factor , programming language
In this paper we describe the cloning and sequencing of the gene ( SUL1 ) responsible for sulphite resistance in a Saccharomyces cerevisiae mutant (Casalone et al. , 1992). The deduced amino acid sequence predicted that the gene codes for a zinc‐finger protein with five fingers. Comparison of wild‐type and mutant gene sequences demonstrated that the mutation event was a transversion from C to G; as a consequence of the mutation a histidine substituted an aspartic acid, affecting directly the fourth finger structure. The SUL1 gene sequence corresponds to that of FZF1 gene (Breitwieser et al. , 1993) to which no function was attributed. The sequence has been entered in the EMBL data library under Accession Number 77592.