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Molecular analysis of the malic enzyme gene ( mae2 ) of Schizosaccharomyces pombe
Author(s) -
Viljoen Marinda,
Subden Ron E.,
Krizus Aldis,
van Vuuren Hendrik J. J.
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100506
Subject(s) - schizosaccharomyces pombe , biology , malic enzyme , gene , nucleic acid sequence , open reading frame , homology (biology) , amino acid , coding region , peptide sequence , biochemistry , genetics , microbiology and biotechnology , enzyme , schizosaccharomyces , saccharomyces cerevisiae , dehydrogenase
Sequence analysis of a 4·6‐kb Hin dIII fragment containing the malic enzyme gene ( mae2 ) of Schizosaccharomyces pombe , revealed the presence of an open reading frame of 1695 nucleotides, coding for a 565 amino acid polypeptide. The mae2 gene is expressed constitutively and encodes a single mRNA transcript of 2·0 kb. The mae2 gene was mapped on chromosome III by chromoblotting. The coding region and inferred amino acid sequence showed significant homology with 12 malic enzyme genes and proteins from widely different origins. Eight highly homologous regions were found in these malic enzymes, suggesting that they contain functionally conserved amino acid sequences that are indispensable for activity of malic enzymes. Two of these regions have previously been reported to be NAD‐ and NADP‐binding sites.