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I . Yeast sequencing reports. Isolation and characterization of the LEU2 gene of Hansenula polymorpha
Author(s) -
Agaphonov Michael. O.,
Poznyakovski Andrei I.,
Bogdanova Aliona I.,
TerAvanesyan Michael D.
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100410
Subject(s) - biology , isolation (microbiology) , yeast , genetics , gene , dna sequencing , microbiology and biotechnology , computational biology
A DNA fragment carrying the LEU2 gene of methylotrophic yeast Hansenula polymorpha was isolated by complementation of the leuB mutation of Escherichia coli . The nucleotide sequence of the isolated DNA fragment contains an open reading frame of 363 codons, coding for a protein 80% identical to the LEU2 gene product of Saccharomyces cerevisiae . Further downstream, there is a partial reading frame with no obvious similarity to known proteins. The LEU2 gene of H. polymorpha cannot complement the leu2 mutation of S. cerevisiae . The sequence has been entered in the EMBL data library under the Accession Number U00889.