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A search for an essential function of the replication origin ARS1 in the life cycle of Saccharomyces cerevisiae
Author(s) -
Kirpekar Finn,
Friis Jørgen,
Gulløv Kay
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100408
Subject(s) - biology , control of chromosome duplication , dna replication , replication (statistics) , origin recognition complex , genetics , licensing factor , pre replication complex , origin of replication , eukaryotic dna replication , saccharomyces cerevisiae , replication factor c , dna replication factor cdt1 , minichromosome maintenance , yeast , mutant , genome , autonomously replicating sequence , dna , gene , virology
We have investigated the significance of the chromosomal replication origin, ARS1 , during the entire life cycle of yeast. This was done by substituting the chromosomal copy with a series of ars1 deletion mutants. It was shown that the ARS1 replication origin is not essential for mitotic or premeiotic DNA replication since no effect on growth, chromosomal loss rate and spore viability was observed in the ars1 mutant strains. We conclude that replication origins are abundantly, present in the yeast genome and that the removal of a single replication origin is compensated for by replication forks emanating from neighbouring origins.