XIII . Yeast sequencing reports. Cloning and sequencing of the  NES24  gene of  Saccharomyces cerevisiae | Zendy

Yoshida Miho | Zendy; Shimma YohIchi | Zendy; Uno Isao | Zendy; TohE Akio | Zendy

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XIII . Yeast sequencing reports. Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae

Author(s) -

Yoshida Miho,

Shimma YohIchi,

Uno Isao,

TohE Akio

Publication year - 1994

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.320100309

Subject(s) - biology , genetics , genbank , gene , open reading frame , homology (biology) , ecori , mutant , xhoi , accession number (library science) , complementation , dna sequencing , cloning (programming) , microbiology and biotechnology , peptide sequence , plasmid , computer science , programming language

We have cloned NES24 using a temperature‐sensitive nes24 ‐ 1 mutant as a host and sequenced a 3162 bp Xho I‐ Eco RI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24 ‐ 1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature‐sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.

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