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XIII . Yeast sequencing reports. Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae
Author(s) -
Yoshida Miho,
Shimma YohIchi,
Uno Isao,
TohE Akio
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100309
Subject(s) - biology , genetics , genbank , gene , open reading frame , homology (biology) , ecori , mutant , xhoi , accession number (library science) , complementation , dna sequencing , cloning (programming) , microbiology and biotechnology , peptide sequence , plasmid , computer science , programming language
We have cloned NES24 using a temperature‐sensitive nes24 ‐ 1 mutant as a host and sequenced a 3162 bp Xho I‐ Eco RI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24 ‐ 1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature‐sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.