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II . Yeast sequencing reports. The sequence of an 8·8 kb segment on the left arm of chromosome II from Saccharomyces cerevisiae reveals four new open reading frames including homologs of animal DNA polymerase α‐primases and bacterial GTP cyclohydrolase II
Author(s) -
van Dyck Luc,
Purnelle Bénédicte,
Goffeau André,
Skala Jacek
Publication year - 1994
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320100003
Subject(s) - biology , genetics , microbiology and biotechnology , cosmid , saccharomyces cerevisiae , dna , gene
The DNA sequence of two contiguous 7648 bp and 1194 bp Bam HI fragments from the cosmid α1201 located about 60 kb from the centromere on the left arm of chromosome II from Saccharomyces cerevisiae has been determined. Sequence analysis reveals four new open reading frames longer than 300 bp: YBL0415 (309 bp), YBL0416 (4539 bp). YBL0417 (1035 bp) and YBL0414 (2115 bp), which extends into the neighbouring 5·2 kb Bam HI fragment. The YBL0414 shows homologies to the mouse 68 kDa and Drosophila melanogaster 76 kDa subunits of the DNA polymerase α‐primase complex. The YBL0417 is homologous to bacterial GTP cyclohydrolase II (EC 3.5.4.25). The sequence has been deposited in the EMBC data library under Accession Number X74738.