Premium
The DNA sequence analysis of the HAP4 – LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast
Author(s) -
Chéret Geneviève,
Pallier Chantal,
Valens Michèle,
DaignanFornier Bertrand,
Fukuhara Hiroshi,
BolotinFukuhara Monique,
Sor Frédéric
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320091113
Subject(s) - orfs , biology , genetics , gene , saccharomyces cerevisiae , nucleic acid sequence , open reading frame , coding region , dna , base pair , chromosome , homology (biology) , peptide sequence , microbiology and biotechnology
The nucleotide sequence of a 19 000 base pair region from the left arm of chromosome XI of Saccharomyces cerevisiae has been determined and analysed. It covers the HAP4–GFA1–LAP4 loci already described. As expected HAP4, GFA1 and LAP4 genes have been found and six new open reading frames (ORFs) with a coding capacity of more than 100 amino acid residues have been identified. One of them (YKL461) shows a high degree of identity with an aspartate aminotransferase gene. This raises the question of a second aspartate aminotransferase gene in yeast. A second ORF (YKL462) shows features compatible with a membranous localization. The other ORFs do not show a similarity with any known gene. A member of the highly repetitive ‘CAT’ DNA sequence is present.