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The 5‐aminoimidazole ribonucleotide‐carboxylase structural gene of the methylotrophic yeast Pichia methanolica : Cloning, sequencing and homology analysis
Author(s) -
Hiep Tran Tuan,
Kulikov Vladimir N.,
Noskov Vladimir N.,
Sizonenko Genadij I.,
Chernoff Yuri O.,
Pavlov Yuri I.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320091112
Subject(s) - biology , ura3 , schizosaccharomyces pombe , genetics , homology (biology) , nucleic acid sequence , gene , open reading frame , complementation , saccharomyces cerevisiae , microbiology and biotechnology , pichia , peptide sequence , biochemistry , pichia pastoris , recombinant dna , mutant
The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl‐5‐aminoimidazole‐carboxylase (AIRC, EC 4.1.1.21), which is involved in purine biosynthesis. The gene was cloned by complementation of an ade2 mutation in Saccharomyces cerevisiae and a 3077 nucleotide DNA fragment was sequenced. The sequence possessed a single open reading frame, corresponding to a 543 amino acid sequence. The sequence of this putative protein has been compared to the proteins of homologous genes from S. cerevisiae, Schizosaccharomyces pombe, Escherichia coli , chicken and man. The analysis revealed remarkable homology between yeast AIRCs, while for other proteins homology was limited to defined regions.