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The ERG3 gene in Saccharomyces cerevisiae is required for the utilization of respiratory substrates and in heme‐deficient cells
Author(s) -
Smith Steven J.,
Parks Leo W.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320091104
Subject(s) - saccharomyces cerevisiae , biology , mutant , ergosterol , heme , yeast , gene , biochemistry , microbiology and biotechnology , enzyme
ERG3 is the structural gene in Saccharomyces cerevisiae for the sterol Δ 5 desaturase that introduces the C5=6 unsaturation in ergosterol biosynthesis. The ERG3 gene has been mapped on chromosome XII, 13·7 centimorgans from GAL2 toward SPT8 . The essentially of the gene is dependent on the conditions used for the cultivation of the mutants. Insertionally inactivated mutants of ERG3 fail to grow without ‘sparking’ levels of Δ 5 sterols in heme‐deficient cells, and are unable to grow on the respiratory substrates glycerol and ethanol.