Premium
The RHO4a and NUD1 genes on Saccharomyces cerevisiae chromosome XI
Author(s) -
Van VlietReedijk Joke C.,
Planta Rudi J.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320091015
Subject(s) - biology , saccharomyces cerevisiae , genetics , gene , chromosome
Sequence analysis of a 4 kb fragment from the right arm of Saccharomyces cerevisiae chromosome XI, in combination with Northern hybridization experiments revealed the presence of two genes, designated RHO4a and NUD1 . The first gene encodes a 32 kDa protein showing significant sequence similarity with members of the ras family. Its 3′‐terminal sequence is virtually identical to a sequence published previously as the RHO4 gene [Matsui and Toh‐e, Gene 114 (1992), 43–49], which, however, appears to start at an internal ATG codon. The RHO4a sequence also overlaps the 5′‐terminal sequence of the RNC1 gene [Chow et al., Nucl. Acids Res. 20 (1992), 5215–5221] proposed to encode the yeast yNucR endo/exonuclease. The remainder of this RNC1 gene overlaps with the 5′‐end of the NUD1 gene. However, the RNC1 sequence lacks a portion of 276 bp that in our fragment is part of the intergenic region separating RHO4a and NUD1 . From these results we conclude that the proposed RNC1 gene is the result of a cloning artefact and that the yNucR protein is instead encoded by the NUD1 gene.