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The nucleotide sequence of a 2·1 kb fragment from chromosome VI of Saccharomyces cerevisiae identifies a tRNA Gly gene, part of a delta element and a palindromic sequence
Author(s) -
Van Heusden G. Paul H.,
De Koning Wim J.,
Van Der Aart Quirina J. M.,
Van Den Berg Johan A.,
Steensma H. Yde
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320091011
Subject(s) - biology , nucleic acid sequence , gene , genetics , palindromic sequence , saccharomyces cerevisiae , microbiology and biotechnology , complementary dna , open reading frame , diacylglycerol kinase , palindrome , peptide sequence , genome , kinase , protein kinase c
The nucleotide sequence was determined of a 2·1 kb DNA fragment located at approximately 35 kb to the right of the centromere of chromosome VI from Saccharomyces cerevisiae . Analysis revealed the presence of a tRNA GLy gene, part of a delta element and a remarkable palindromic sequence. The longest open reading frame found encodes a putative protein of 195 amino acids. Although the fragment was isolated by hybridization to a human diacylglycerol kinase cDNA, no evidence was obtained for the presence of a gene encoding diacylglycerol kinase.