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Cloning and sequence of ADP‐ribosylation factor 1 (ARF1) from Schizosaccharomyces pombe
Author(s) -
Erickson F. Les.,
Hannig Ernest M.,
Krasinskas Alyssa,
Kahn Richard A.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090812
Subject(s) - schizosaccharomyces pombe , biology , saccharomyces cerevisiae , adp ribosylation factor , gene , schizosaccharomyces , complementary dna , cloning (programming) , genetics , cdna library , context (archaeology) , paleontology , cell , computer science , programming language , golgi apparatus
A gene encoding a homologue of the ADP‐ribosylation factor (ARF) family of small GTP binding proteins was cloned from a Schizosaccharomyces pombe cDNA library by a functional screen of suppressors of sensitivity to 3‐aminotriazole in a gcn3 null strain of Saccharomyces cerevisiae. Two independent isolates each contained the full coding region of the ARF1 gene. The encoded SpARF1 protein has a predicted molecular weight of 20 618 and is 88% and 79% identical to human and S. cerevisiae ARF1 proteins, respectively. As independent isolates were obtained, this effect of the SpARF1 appears to be a real phenomenon, but cannot currently be easily understood within the context of the evidence for a role(s) for ARF proteins in the protein secretory pathway.