Cloning and sequence of ADP‐ribosylation factor 1 (ARF1) from  Schizosaccharomyces pombe | Zendy

Erickson F. Les. | Zendy; Hannig Ernest M. | Zendy; Krasinskas Alyssa | Zendy; Kahn Richard A. | Zendy

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Cloning and sequence of ADP‐ribosylation factor 1 (ARF1) from Schizosaccharomyces pombe

Author(s) -

Erickson F. Les.,

Hannig Ernest M.,

Krasinskas Alyssa,

Kahn Richard A.

Publication year - 1993

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.320090812

Subject(s) - schizosaccharomyces pombe , biology , saccharomyces cerevisiae , adp ribosylation factor , gene , schizosaccharomyces , complementary dna , cloning (programming) , genetics , cdna library , context (archaeology) , paleontology , cell , computer science , programming language , golgi apparatus

A gene encoding a homologue of the ADP‐ribosylation factor (ARF) family of small GTP binding proteins was cloned from a Schizosaccharomyces pombe cDNA library by a functional screen of suppressors of sensitivity to 3‐aminotriazole in a gcn3 null strain of Saccharomyces cerevisiae. Two independent isolates each contained the full coding region of the ARF1 gene. The encoded SpARF1 protein has a predicted molecular weight of 20 618 and is 88% and 79% identical to human and S. cerevisiae ARF1 proteins, respectively. As independent isolates were obtained, this effect of the SpARF1 appears to be a real phenomenon, but cannot currently be easily understood within the context of the evidence for a role(s) for ARF proteins in the protein secretory pathway.

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