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A 12·8 kb segment, on the right arm of chromosome II from Saccharomyces cerevisiae including part of the DUR1, 2 gene, contains five putative new genes
Author(s) -
Bussereau Françoise,
Mallet Laurent,
Jacquet Michel,
Gaillon Laurent
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090714
Subject(s) - biology , orfs , genetics , gene , saccharomyces cerevisiae , schizosaccharomyces pombe , open reading frame , nucleic acid sequence , autonomously replicating sequence , homology (biology) , peptide sequence , microbiology and biotechnology , plasmid , origin of replication
A 12 820 bp fragment from the right arm of chromosome II of Saccharomyces cerevisiae was sequenced and analysed. This fragment contains six non‐overlapping long open reading frames (ORFs) designated from the centromere‐ to the telomere‐proximal ends as: YBR1441, 1443, 1444, 1445, 1446 and 1448. YBR1441 encodes a polypeptide of 845 amino acids which shares a long consensus domain with products of S. cerevisiae MCM2, MCM3, CDC46 and Schizosaccharomyces pombe cdc21 + genes. These genes are involved in DNA replication. YBR1445 encodes a polypeptide of 404 amino acids which has strong similarity with the S. cerevisiae KRE2/MNT1, YUR1, KTR1 gene products. The KRE2/MNT1 protein is an α‐1,2‐mannosyltransferase. The product of YBR1444, which encodes a protein of 375 amino acids, presents a lipase signature sequence and a peroxisomal targeting signal. YBR1448, whose sequence extends further on the telomere‐proximal end of the fragment, is identical to the 3′ end of the DUR1,2 gene encoding urea amidolyase. The two ORFs, YBR1443 and YBR1446, exhibit no significant similarity with any known gene.

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