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Vector YFRp1 allows transformant selection in Saccharomyces cerevisiae via resistance to formaldehyde
Author(s) -
Wehner Eugen P.,
Brendel Martin
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090712
Subject(s) - yeast , auxotrophy , saccharomyces cerevisiae , biology , transformation (genetics) , gene , formaldehyde , vector (molecular biology) , shuttle vector , biochemistry , recombinant dna , mutant
Formaldehyde (FA), a chemical with low toxic potential, is used as sole selective agent for transformation in the yeast Saccharomyces cerevisiae . Neither stable auxotrophic markers in recipient cells nor defined synthetic media are needed when multicopy vector YFRp1, containing the yeast SFA gene, is employed for yeast transformation. The SFA gene gives stability to the vector and its yeast (and other) passenger genes when transformants are propagated in complex media supplemented with 3–5 mM‐FA. Use of inexpensive FA and non‐synthetic, undefined media will lower the cost of yeast transformant propagation considerably and thus make feasible large‐volume industrial application of transformants containing YFRp1 derivatives.