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The yal017 gene on the left arm of chromosome I of Saccharomyces cerevisiae encodes a putative serine/threonine protein kinase
Author(s) -
Clark Michael W.,
Zhong Wu Wei,
Ouellette B. F. Francis,
Bussey Howard,
Keng Teresa,
Storms Reginald K.,
Barton Arnold,
Kaback David B.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090511
Subject(s) - biology , c raf , akt3 , map2k7 , open reading frame , protein serine threonine kinases , threonine , kinase , protein kinase a , biochemistry , microbiology and biotechnology , gene , serine , casein kinase 2 , cyclin dependent kinase 2 , peptide sequence , phosphorylation
The DNA sequence of a region between the LTE1 and CYS3 genes on the left arm of chromosome I from Saccharomyces cerevisiae contains an open reading frame (ORF), YAL017 , corresponding to the 5·0 kb FUN31 ( F unction U nknown N ow) transcribed region. The predicted protein from this ORF contains 1358 amino acid residues with a molecular weight of 152 531, and an identifiable serine/threonine protein kinase catalytic domain. When compared with other yeast protein kinases, the Ya1017p kinase most resembles the SNF1 serine/threonine protein kinase which is involved in regulating sucrose fermentation genes. The Ya1017p kinase shows highest amino acid identities with two mammalian carcinoma‐related serine/threonine protein kinases; PIM ‐ 1 , which shows induced expression in T‐cell lymphomas; and p78A1, whose expression is lost in human pancreatic carcinomas. Gene disruption of YAL017 indicates that it is non‐essential for growth on glucose.