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Physical localization of yeast CYS3 , a gene whose product resembles the rat γ‐cystathionase and Escherichia coli cystathionine γ‐synthase enzymes
Author(s) -
Barton Arnold B.,
Kaback David B.,
Clark Michael W.,
Keng Teresa,
Ouellette B. F. Francis,
Storms Reg K.,
Zeng Bin,
Zhong Wuwei,
Fortin Nathalie,
Delaney Shawn,
Bussey Howard
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090406
Subject(s) - cystathionine beta synthase , biology , gene , auxotrophy , cysteine , gene product , escherichia coli , homology (biology) , biochemistry , locus (genetics) , enzyme , saccharomyces cerevisiae , cystathionine gamma lyase , genetics , structural gene , yeast , microbiology and biotechnology , gene expression
We have cloned, sequenced and physically mapped the CYS3 gene of Saccharomyces cerevisiae . This gene can complement the cys3–1 allele, and disruptions at this locus lead to cysteine auxotrophy. The predicted CYS3 product is closely related (46% identical) to the rat cystathionine γ‐lyase (Erickson et al. , 1990), but differs in lacking cysteine residues. These results provide further evidence that the S288C strain of yeast resembles mammals in synthesizing cysteine solely via a trans ‐sulfuration pathway. The CYS3 product was found to have strong homology to three other enzymes involved in cysteine metabolism: the Escherichia coli metB and metC products and the S. cerevisiae MET25 gene product. The trans ‐sulfuration enzymes appears to form a diverged family and carry out related functions from bacteria to mammals.