Physical localization of yeast  CYS3 , a gene whose product resembles the rat γ‐cystathionase and  Escherichia coli  cystathionine γ‐synthase enzymes | Zendy

Barton Arnold B. | Zendy; Kaback David B. | Zendy; Clark Michael W. | Zendy; Keng Teresa | Zendy; Ouellette B. F. Francis | Zendy; Storms Reg K. | Zendy; Zeng Bin | Zendy; Zhong Wuwei | Zendy; Fortin Nathalie | Zendy; Delaney Shawn | Zendy; Bussey Howard | Zendy

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Physical localization of yeast CYS3 , a gene whose product resembles the rat γ‐cystathionase and Escherichia coli cystathionine γ‐synthase enzymes

Author(s) -

Barton Arnold B.,

Kaback David B.,

Clark Michael W.,

Keng Teresa,

Ouellette B. F. Francis,

Storms Reg K.,

Zeng Bin,

Zhong Wuwei,

Fortin Nathalie,

Delaney Shawn,

Bussey Howard

Publication year - 1993

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.320090406

Subject(s) - cystathionine beta synthase , biology , gene , auxotrophy , cysteine , gene product , escherichia coli , homology (biology) , biochemistry , locus (genetics) , enzyme , saccharomyces cerevisiae , cystathionine gamma lyase , genetics , structural gene , yeast , microbiology and biotechnology , gene expression

We have cloned, sequenced and physically mapped the CYS3 gene of Saccharomyces cerevisiae . This gene can complement the cys3–1 allele, and disruptions at this locus lead to cysteine auxotrophy. The predicted CYS3 product is closely related (46% identical) to the rat cystathionine γ‐lyase (Erickson et al. , 1990), but differs in lacking cysteine residues. These results provide further evidence that the S288C strain of yeast resembles mammals in synthesizing cysteine solely via a trans ‐sulfuration pathway. The CYS3 product was found to have strong homology to three other enzymes involved in cysteine metabolism: the Escherichia coli metB and metC products and the S. cerevisiae MET25 gene product. The trans ‐sulfuration enzymes appears to form a diverged family and carry out related functions from bacteria to mammals.

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