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Characterization of plasma membrane H + ‐ATPase from salt‐tolerant yeast Candida versatilis
Author(s) -
Watanabe Yasuo,
Yamaguchi Masahiro,
Sakamoto Jun,
Tamai Youichi
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090302
Subject(s) - atpase , biology , saccharomyces cerevisiae , membrane , biochemistry , enzyme , in vivo , ammonium sulfate , yeast , stimulation , microbiology and biotechnology , chemistry , chromatography , endocrinology
Plasma membrane was isolated from the salt‐tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H + ‐ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H + ‐ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH 4 + , especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii . No in vivo activation of H + ‐ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells.