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Change in transport activities of vacuoles of the yeast Yarrowia lipolytica during its growth on glucose
Author(s) -
Kulakovskaya T. V.,
Matyashova R. N.,
Shishkanova N. V.,
Finogenova T. V.,
Okorokov L. A.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090203
Subject(s) - yarrowia , vacuole , yeast , biology , biochemistry , efflux , growth medium , atpase , enzyme , bacteria , genetics , cytoplasm
Vacuoles were isolated from Yarrowia lipolytica yeast cells taken at various growth phases under carbon or nitrogen limitation. Vacuoles from the cells at the logarithmic growth phase showed a high activity of vacuolar H + ‐ATPase (0·9–1·1 U/mg protein) and efficiently generated chemical proton gradient and membrane potential across the tonoplast. Ca 2+ ‐ and citrate transport were found to be maximal at this growth phase. At growth retardation and then in the stationary phase all the parameters studied decreased irrespective of the method of growth limitation. The citrate‐transporting activity of vacuoles completely disappeared at growth retardation, also irrespective of the limitation method and irrespective of whether yeast cells overproduced citrate in the culture medium. The citrate‐transporting system of Y. lipolytica vacuolar membrane is concluded not to be involved in citrate efflux and this efflux is probably performed by the plasmalemma transport system.