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In Saccharomyces cerevisiae , protein secretion into the growth medium depends on environmental factors
Author(s) -
Rossini Dominique,
Porro Danilo,
Brambilla Luca,
Venturini Marina,
Ranzi Bianca Maria,
Vai Marco,
Alberghina Lilia
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090110
Subject(s) - saccharomyces cerevisiae , excretion , biology , heterologous , yeast , secretion , biochemistry , escherichia coli , growth medium , bacteria , genetics , gene
In the budding yeast Saccharomyces cerevisiae the cell wall, mainly composed of mannoproteins and glucans, constitutes a barrier to protein excretion in the growth medium. In this paper we have studied the effects of different environmental parameters on excretion of Escherichia coli β‐galactosidase obtained by exploiting the glucoamylase II signal sequence. Excretion of the unglycosylated β‐galactosidase was detectable only in cells grown in rich medium, was affected by temperature (36°C > 30°C >> 24°C) and slightly stimulated by reducing agents. On the contrary, glycosylated proteins, such as α‐galactosidase and glucoamylase II, were excreted to a good extent under all tested conditions of medium composition, growth temperature and pH. These data indicate that optimization of environmental parameters may help the excretion of heterologous proteins, offering advantages for protein purification.