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A yeast antiviral protein, SKI8 , shares a repeated amino acid sequence pattern with β‐subunits of G proteins and several other proteins
Author(s) -
Matsumoto Yutaka,
Sarkar Gobinda,
Sommer Steve S.,
Wickner Reed B.
Publication year - 1993
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320090106
Subject(s) - biology , yeast , peptide sequence , sequence (biology) , protein subunit , amino acid , biochemistry , genetics , computational biology , gene
SKI8 is a yeast antiviral gene, essential for controlling the propagation of M double‐stranded RNA (dsRNA) and thus for preventing virus‐induced cytopathology. Our DNA sequence of SKI8 shows that it encodes a 397 amino acid protein containing two copies of a 31 amino acid repeat pattern first identified in mammalian β‐transducin and Cdc4p of yeast. There are also four copies of this repeat in yeast Mak11p, necessary for M dsRNA propagation, and three copies in the putative product of the Dictyostelium AAC3 gene. Analysis of 36 cases of the repeat unit shows they have a consensus predicted structure: N–helix–sheet–turn–sheet–turn–sheet–helix–C.