Premium
The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical
Author(s) -
Yoo Hyang Sook,
Cunningham Thomas S.,
Cooper Terrance G.
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320081202
Subject(s) - permease , biology , allantoin , saccharomyces cerevisiae , gene , biochemistry , genetics , lactose permease , homologous chromosome , catabolite repression , mutant
We have determined the structure of the allantoin permease ( DAL4 ) gene of Saccharomyces cerevisiae . The gene patatively encodes a hydrophobic protein with a M r of 71 755. It possesses the alternating hydrophobic–hydrophilic regions similar to those found in many other integral membrane proteins. The most striking feature of the allantoin permease component encoded by DAL4 is its striking similarity to the uracil permease component encoded by FUR4 Although data available indicate that these proteins do not share any overlap of function, their predicted protein sequences are 68% identical, 81% similar, and their DNA sequences are 70% identical. The upstream regulatory region of DAL4 contains all lof the characterized cis ‐acting elements previously reported for inducible allantoin pathway genes: six sequences homologous to UAS NTR , the element responsible for nitrogen catabolite repression‐sensitive activation of allantoin pathway gene expression, and two sequences homologous to the cis ‐acting element responsible for inducere‐responsiveness of the allantoin pathway genes, UIS . The finding of these homologous sequences predicted to exist on the basis of DAL4 's expression characteristics, supports and strengthens the suggestion that these elements mediate the functions we have we have previously ascribed to them.