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Structure and expression of the ABF1‐regulated ribosomal protein S33 gene in Kluyveromyces
Author(s) -
Hoekstra Ruurdtje,
Ferreira Pedro Moradas,
Bootsman Theo C.,
Mager Willem H.,
Plnata Rudi J.
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320081105
Subject(s) - biology , kluyveromyces , gene , ribosomal protein , gene expression , ribosomal rna , genetics , microbiology and biotechnology , computational biology , ribosome , rna , saccharomyces cerevisiae
The abundant multifuctional protein ABF1 of Saccharomyces cerevisiae binds to the upstream region of several genes, including some ribosomal protein genes like the one encoding protein S33. Deletion of th ABF1‐binding sequence lowers the transcription of these genes three‐ to more than ten‐fold. We have isolated the S33 genes of two related yeast species. Kluyveromyces lactis and Kluveromyces marxianus . Comparison of the nucleotide sequences of these S33 genes with their counterpart form S. Cerevisiae shows a strong sequence similarity covering the whole of the coding regions. In contrast, little or no sequence similarly is found in the 5′‐flanking regions of the three genes. Also the trailer regions differ considerably in both length and sequence from one species to another. An ABF1‐binding site is present in the upstream region of the S33 gene of K. marxianus . Retardation analyses showed that this sequences is able to bind a protein present in Kluyveromyces cells with a molecular mass somewhat lower than that of S. cerevisiae ABF1. Functional analyses, using a β‐glucuronidase reporter system, showed that the ABF1‐binding site is indeed involved in transcription activation of the K. marxianus S33 gene in Kluyveromyces DNA and Northern blots did not show a signal. These results indicate that S. cerevisiae and Kluyveromyces contain functionally related but structurally dissimilar ABF1‐type proteins.