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Yeast sequencing reports. LEU2 gene homolog in Kluyveromyces lactis
Author(s) -
Zhang YingPei,
Chen XinJie,
Li YuYang,
Fukuhara Hiroshi
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080914
Subject(s) - kluyveromyces lactis , biology , open reading frame , genetics , gene , nucleic acid sequence , saccharomyces cerevisiae , plasmid , genomic dna , kluyveromyces , microbiology and biotechnology , peptide sequence
A DNA fragment that can complement the leu2 mutation of Saccharomyces cerevisiae was cloned from the genomic library of Kluyveromyces lactis . The nucleotide sequence revealed an open reading frame of 362 codons, 75% homologous to S. cerevisiae LEU2 gene. The upstream region contained a CCGGAACCGG sequence identical to the site of leucine‐specific control of LEU2. Further upstream, there is a partial open reading frame homologous to rat ribosmal protien L7.