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Yeast sequencing reports. Sequence of the genes encoding subunits A and B of the vacuolar H + ‐ATPase of Schizosaccharomyces pombe
Author(s) -
Ghislain Michel,
Bowman Emma Jean
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080913
Subject(s) - schizosaccharomyces pombe , neurospora crassa , biology , gene , intron , genetics , schizosaccharomyces , saccharomyces cerevisiae , protein subunit , coding region , neurospora , conserved sequence , gene product , microbiology and biotechnology , peptide sequence , gene expression , mutant
The genes coding subunits A (vma1) and B (vma2) of the vacuolar H + ‐ATPase from Schizosaccharomyces pombe were cloned by hybridization to cDNAs of the homologous genes in Neurospora crasa . Both genes are interrupted by introns, two in vma1 and four in vma2 . Positions of introns do not appear to be conserved when compared to those of N. crassa . The subunit A gene encodes a single product of 619 amino acids and is not interrupted by the coding sequence for a second product as found for Saccharomyces cerevisiae (Kane, P.K., Yamashiro, C.T., Wolczyk, D.F., Neff, N., Goebl, M., and Stevens, T.H. (1990). Science 250 , 651–657).