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Rag − mutations involved in glucose metabolism in yeast: Isolation and genetic characterization
Author(s) -
WésolowskiLouvel M.,
Prior C.,
Bornecque D.,
Fukuhara H.
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080904
Subject(s) - biology , complementation , kluyveromyces lactis , mutant , phenotype , kluyveromyces , gene , yeast , genetics , mutation , nuclear gene , allele , biochemistry , saccharomyces cerevisiae , microbiology and biotechnology , mitochondrial dna
Some natural isolates and many laboratory strains of the yeast Kluyveromyces lactis cannot grow on glucose when respiration is inhibited by antimycin A. The ability or inability to grow on glucose in the absence of mitochondrial respiration has been called Rag + or Rag − phenotype (resistance to antimycin on glucose, respectively). Rag − strains, unable to grow on glucose in the presence of the respiratory drug, behave as if they were defective in fermentation. The Rag phenotype was first found to be determined by variant alleles of either of the two nuclear genes, RAG1 and RAG2 , which code for a low‐affinity glucose transport protein and for phosphoglucose isomerase, respectively. These findings suggested that the Rag − phenotype can be used to obtain mutations of genes involved in glucose metabolism in K. lactis. We thus looked for other Rag − mutants. Seventy‐four mutants were isolated and genetically characterized. All of the mutations were nuclear recessive alleles, defining 11 new complementation groups, which we designate rag3 through rag13 .