Premium
Efficient electropulse transformation of intact Candida maltosa cells by different homologous vector plusmids
Author(s) -
Kasüske Anette,
Wedler Holger,
Schulze Steffen,
Becher Dietmar
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080902
Subject(s) - electroporation , plasmid , transformation (genetics) , transformation efficiency , biology , dna , homologous chromosome , microbiology and biotechnology , recombinant dna , vector (molecular biology) , strain (injury) , genetics , gene , agrobacterium , anatomy
Conditions for efficient and quick transformation by electroporation were developed in Candida maltosa . To investigate the efficiency of transformation with integrative as well as with autonomously replicating plasmids, a series of vectors was constructed for homologous transformation of the species. Transformants were obtained with different plasmids as covalently closed circular molecules and as linearized DNA. The influence of recipient strain and plasmid type as well as of cell number and parameters of the supplied electrical pulse on the transformation efficiency have been investigated. A maximum of 7000 transformants per 100ng of plasmid DNA was reached. The efficiency of transformation was compared with that of the LiCl method.