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Dosage‐dependent translational suppression in yeast Saccharomyces cerevisiae
Author(s) -
Chernoff Yury O.,
IngeVechtomov Sergey G.,
Derkach Irina L.,
Ptyushkina Marina V.,
Tarunina Olga V.,
Dagkesamanskaya Adilya R.,
TerAvanesyan Michael D.
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080702
Subject(s) - extrachromosomal dna , biology , saccharomyces cerevisiae , gene , plasmid , yeast , genetics , mutant , coding region
The overexpression of SUP35 ( SUP2 ) wild‐type gene, caused by increase of its copy number, induces an omnipotent suppression similar to the phenotype of mutants for this gene. The effectt of extra‐ SUP35 was detected for moderate or even low copy number. Moreover, overdosage of the fragment including only the 5′‐flanking region and N‐terminal 100 bp of protein‐coding sequence of SUP35 leads to allosuppression. Multi‐ SUP35 gene was also icompatible with extrachromosomal suppressor factor ψ, presumbaly because of high level of mistranslation. The suppressor effect caused by overdosage of another gene, SUP45 ( SUP1 ), is much lower and can be detected only for one construction which is derived from high copy number plasmid. Suppression induced by extra‐ SUP35 and especially by extra‐ SUP45 is affected by the cell environment. A model predicting that the balance of gene products is a key for regulation of translational fidelity is discussed.