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The complete sequence of a 10·8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae
Author(s) -
Biteau Nicolas,
Fremaux Christophe,
Hebrard Sylvie,
Menara Annie,
Aigle Michael,
Crouzet Marc
Publication year - 1992
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320080107
Subject(s) - orfs , biology , centromere , genetics , gene , open reading frame , nucleic acid sequence , fragment (logic) , saccharomyces cerevisiae , chromosome , microbiology and biotechnology , peptide sequence , computer science , programming language
The complete nucleotide sequence of the D10H fragment (10850 bp) was determined. The D10H fragment is located on the right arm of chromosome III near the centromere and contains the SUF2 gene. Six open reading frames (ORFs) larger than 300 bp were found. One of them is the CIT2 gene encoding the cytoplasmic citrate synthase. The others are new putative genes and show no significant similarly with any known gene. In addition two tRNA genes (Asn and Pro) and a solo delta element were identified. Two ORFs were disrupted; no peculier phenotype was observed.