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TDH2 is linked to MET3 on chromosome X of Saccharomyces cerevisiae
Author(s) -
Mountain Harry A.,
Korch Christopher
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070814
Subject(s) - biology , genetics , clone (java method) , gene , restriction map , nucleic acid sequence , southern blot , cloning (programming) , saccharomyces cerevisiae , microbiology and biotechnology , molecular cloning , chromosome , genomic dna , peptide sequence , computer science , programming language
The MET3 gene of Saccharomyces cerevisiae was cloned and its restriction map was found to differ in the upstream region from an earlier published map (Cherest et al. Gene 34 , 269–281, 1985) and nucleotide sequence (Cherest et al. Mol. Gen. Genet . 210 , 307–313, 1987). Southern blot analysis of genomic DNA from strains S288C and FL100 (the genetic backgrounds from which these different copies of the gene had been cloned) showed that our clone from a S288C‐based library had the same restriction map as the chromosomal DNA from both of the strains. Comarison of the nucleotide sequence of the two clones indicated that the earlier published clone probably represented a cloning artifact. In our clone, we found upstream of MET3 , the nucleotide sequence of the TDH2 gene (Holland and Holland, J. Biol. Chem . 255 , 2596–2605, 1980). The chromosomal orientation of the two genes was determined to be MET3 – TDH2 – CEN10 .