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The sequence of 8·8 kb of yeast chromosome III cloned in lambda PM3270 contains an unusual long ORF (YCR601)
Author(s) -
Rodriguez Francesco,
Martegani Enzo,
Mauri Isabella,
Alberghina Lilia
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070612
Subject(s) - biology , open reading frame , genetics , homology (biology) , nucleic acid sequence , gene , microbiology and biotechnology , polyadenylation , clone (java method) , chromosome , peptide sequence , messenger rna
We have determined the nucleotide sequence of a segment of chromosome III contained in the right part of the lambda PM3270 clone, for a total of 8824 bp. This sequence contains an unusual long open reading frame, YCR601, of 6501 bp that encodes for a protein of 2167 amino acids that show no homology with other known proteins. YCR601 was disrupted by internal deletion and insertion of LEU2 gene and is a non‐essential gene, however it is transcribed during vegetative growth yielding a polyadenylated mRNA of approximately 7 kb.