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Incorporation of unsaturated fatty acids by Saccharomyces cerevisiae : Conservation of fatty‐acyl saturation in phosphatidylinositol
Author(s) -
Pilkington Bridget J.,
Rose Anthony H.
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070508
Subject(s) - biology , saccharomyces cerevisiae , saturation (graph theory) , phosphatidylinositol , biochemistry , yeast , kinase , mathematics , combinatorics
Saccharomyces cerevisiae was grown anaerobically in media supplemented with myritoleic 14:1(9 c ), palmitoleic 16:1(9 c ), oleic 18:1(9 c ), linoleic 18:2(9,12 c ), γ‐linolenic 18:3(9,12,15 c ) or eicosenoic 20:1(11 c ) acid. Cells from exponential‐phase cultures contained approximately the same proportions of the major phospholipid classes, namely phosphatidycholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, the greatest differences being detected in cells grown in the presence of 14:1(9 c ) or 20:1(11 c ) acids. The extent to which phospholipids from cells were enriched with residues of the exogenously supplied acid varied from 52% in cells grown in the presence of 14:1(9 c ) acid to 13% in cells grown in media supplemented with 20:1(11 c ) acid. Analysis of the fatty‐acyl composition of the four major phospholipid classes revealed that the degree of unsaturation varied considerably in three of the classes, while phosphatidylinositol conserved a high degree of saturation. The possible significance of the latter finding in relation to the physiological role of phosphatidylinositol in the plasma membrane is discussed.

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