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The YDp plasmids: A uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae
Author(s) -
Berben Gilbert,
Dumont Jacques,
Gilliquet Véronique,
Bolle PaulAndré,
Hilger François
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070506
Subject(s) - ura3 , plasmid , selectable marker , biology , saccharomyces cerevisiae , genetics , multiple cloning site , gene , cloning (programming) , computational biology , yeast , vector (molecular biology) , recombinant dna , computer science , programming language
The YDp plasmids ( Y east D isruption p lasmids) are pUC9 vectors bearing a set of yeast gene disruption cassettes, all uniform in structure and differing only in the selectable marker used ( HIS3 , LEU2 , LYS2 , TRP1 or URA3 ). The markers, surrounded by translational termination codons, are embedded in the slightly modified sequence of the pUC9 multiple cloning sites.