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Two genes encoding putative mitochondrial alcohol dehydrogenases are present in the yeast Kluyveromyces lactis
Author(s) -
Saliola Michele,
Gonnella Roberta,
Mazzoni Cristina,
Falcone Claudio
Publication year - 1991
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320070409
Subject(s) - kluyveromyces lactis , biology , gene , saccharomyces cerevisiae , kluyveromyces , homology (biology) , genetics , alcohol dehydrogenase , yeast , isozyme , amino acid , biochemistry , nucleic acid sequence , enzyme
Four structural genes encoding isozymes of the alcohol dehydrogenase (ADH) system in the yeast Kluyveromyces lactis have been identified by hybridization to ADH2 DNA probes from Saccharomyces cerevisiae . In this paper we report on the isolation of KlADH4 and the complete sequencing of KlADH3 and KlADH4 , two genes which show high homology to KlADH1 , the ADH gene previously isolated in K. lactis , and to the ADH genes of S. cerevisiae . When compared with KlADH1 , both KlADH3 and KlADH4 encode amino‐terminal extensions which show the characteristics of the mitochondrial targeting sequences. These extensions are poorly conserved both at the nucleotide and the amino acid level. Suprisingly, the KlADH4 extension shows a higher identity at the amino acid level to the one encoded by ADH3 of S. cerevisiae than to the KlADH3 presequence. KlADH3 and KlADH4 , in contrast to the ADH3 gene of S. cerevisiae , show a strong bias in the choice of codons.